Targeting both rs12979860 and rs8099917 polymorphisms with a single-tube high-resolution melting assay for IL28b genotyping

Kok Siong Poon; Sherry Sze Yee Ho; Julian Wei-Tze Tang; Cui Wen Chua; Lily Chiu; Evelyn Siew-Chuan Koay

doi:10.1128/JCM.01718-12

Targeting both rs12979860 and rs8099917 polymorphisms with a single-tube high-resolution melting assay for IL28b genotyping

J Clin Microbiol. 2012 Oct;50(10):3353-5. doi: 10.1128/JCM.01718-12. Epub 2012 Jul 18.

Authors

Kok Siong Poon¹, Sherry Sze Yee Ho, Julian Wei-Tze Tang, Cui Wen Chua, Lily Chiu, Evelyn Siew-Chuan Koay

Affiliation

¹ Molecular Diagnosis Centre, Department of Laboratory Medicine, National University Health System, Singapore.

Abstract

A rapid, duplex, high-resolution melting interleukin-28B gene (IL28B) genotyping assay, targeting both rs12979860 and rs8099917 polymorphisms, was developed and validated using 30 DNA samples from healthy volunteers. A linkage study on 300 healthy Singaporeans showed variable haplotypes. When the assay was applied to plasma DNA from 50 hepatitis C virus genotype-1 (HCV-1)-infected patients, five compound heterozygous types were detected.

Publication types

Evaluation Study

MeSH terms

Clinical Laboratory Techniques / methods*
Genotype
Haplotypes
Hepacivirus / classification
Hepacivirus / genetics
Hepacivirus / isolation & purification
Hepatitis C / drug therapy*
Hepatitis C / virology
Humans
Interferons
Interleukins / genetics*
Molecular Diagnostic Techniques / methods*
Nucleic Acid Denaturation
Polymorphism, Single Nucleotide*
Singapore
Transition Temperature

Substances

interferon-lambda, human
Interleukins
Interferons